Tg(BCL2/IGH)M23Tjd

A human genomic BCL2/IGH fusion minigene was used for the transgene. This construct represents a translocation mutation, t(14:18), found in human follicular B cell lymphomas, and includes 3 amino acid mutations found in human B cell lymphoma line SU-DHL-6. Because of the large size of BCL2 intron 2 (350 kb), a cDNA segment of the exon II/exon III junction was substituted. Transgene expression in the spleen and thymus was confirmed by S1 nuclease protection assay. (J:81147)