来自villin 1基因的12.4千碱基序驱动表达cre重组酶cDNA,包括金属硫蛋白序列和poly(A)信号。重组发生在小肠和大肠的绒毛和隐窝细胞中,精确调控了内源基因的表达。共产生了七条转基因品系。根据作者J:78815所述,表达通常是连续的,但在结肠中观察到轻微的嵌合现象。转基因表达开始于12.5日胚胎干期,比内源性mouse Vil基因的表达开始时间(9.0日胚胎干期)晚。品系997中,转录本插入了17号染色体,位于St6gal2(chr17:55,466,255;NCBI37/mm9)大约119千碱基上游,整合位点伴有14.6千碱基的缺失。(J:78815)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6J x SJL)F1
--
Insertion, Intergenic deletion
--
--
--
638

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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