铁结合域通过插入一个尼姆霉素选择性 cassette,取代了5号到9号染色体的一部分,被破坏了。对杂合突变小鼠产生的牛奶进行 Western blot 分析,未检测到该蛋白。(来源:J:81005)
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The iron binding domain was disrupted with a neomcycin selection cassette that replaced a portion of exon 5 through exon 9. Protein was undetected by Western blot analysis of milk obtained from homozygous mutant mice. (J:81005)