一个包含1.4kb基因组片段,包括1和2号外显子的区域,通过同源重组被替换了neo标记的选择性 cassette。在纯合突变胚胎的提取物中,通过 Western blot 检测未发现蛋白质。(来源:J:88120)
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A 1.4 kb genomic fragment encompassing exons 1 and 2 was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant embryo lysates. (J:88120)