Tg(Cd4-SKP1A)1Ingh

The transgene consists of a mouse Cd4 promoter and a constitutively expressed flag-tagged human SKP1A sequence. Mouse Cd4 regulatory sequences were used to drive transgene expression in T lymphocytes. The Cd4 regulatory region includes the minimal enhancer, minimal promoter, 70 bp of exon 1, and part of intron 1. The Cd4 promoter lacks the silencer region and is transcriptionally active in both single- and double-positive T cells. Transgene expression was confirmed by Western blot analysis of thymocyte extracts from transgenic animals. (J:80320)