整个编码区域被插入了通过同源重组插入的诺美西林抗性基因 cassette,通过西区 blot 对野生型小鼠大脑匀浆检测,未发现蛋白质表达(来源:J:68187)
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The entire coding region was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected in homozygous mutant mice by Wesetern blot analysis of brain homogenates. (J:68187)