Del(17Abcg5-Abcg8)1Hobb

Both the Abcg5 and Abcg8 endogenous loci, which are oriented head to head and separated by approximately 400 bp, were disrupted by the integration of a single targeting construct. A neomycin selection cassette flanked by both loxP and frt sites replaced sequence extending from Abcg5 intron 2 into Abcg8 intron 3. The Walker A consensus sequences that make up a portion of the ATP binding sites in both genes were deleted. Northern and Western blot analyses indicated an absence of normal transcript and protein derived from both loci in homozygous mutant mice. (J:80679)