Tg(LCKprBCL2)36Sjk

The transgene consists of 0.75 kb of Lck proximal promoter sequences (active in lymphoid tissues) and 3.2 kb of human BCL2 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of the construct. Transgene expression in the thymus, lymph nodes, and spleen was verified by Western blot analysis and immunohistochemistry. Seven transgenic lines were established with lines 7, 17, and 36 expressing the transgene at high levels. (J:11548)