Tg(APP)8.9Btla

A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intact transgene. Quantitative RT-PCR analysis of transgenic and wild-type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. (J:80947)