Tg(Igh-6)1Msn

An 11 kb construct containing a rearranged Igh-V region (a allotype) encoding a 4-hydroxy-3-nitrophenenacetyl antibody under the control of the Igh-V promoter was generated using molecular techniques. The construct was designed such that the exons encoding the Igh-6 secretory isoform were included while the exon encoding the membrane isoform was deleted. FACS analysis confirmed a lack of transgenic surface expression, while IgM comprised of transgenic Igh-6 was detected in the serum. (J:80656)