整个编码区域通过同源重组被PGK-neo cassette替换,通过 Western blot 和 homozygous突变小鼠的免疫组化分析,证实了内源性蛋白的缺失(来源:J:75781)
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The entire coding region was replaced with a PGK-neo cassette via homologous recombination. The absence of endogenous protein was demonstrated by Western blot and immunohistochemical analyses of homozygous mutant mice. (J:75781)