包含起始密码子的168bp外显子被通过同源重组插入了抗氨苄青霉素选择标记。通过 Western blot 对纯合突变小鼠的分析,未检测到蛋白质。(来源:J:74969)
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The 168 bp exon containing the translational start codon was replaced with a neomycin selection cassette inserted by homologous recombination. No protein was detected by Western blot analysis of homozygous mutant mice. (J:74969)