在3号和4号外显子被通过同源重组插入了诺美西林选择标记 cassette,删除区域编码了46到144位的氨基酸。通过 Western blot 分析,没有在纯合突变小鼠中检测到蛋白质。(来源:J:58332)
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Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded amino acids 46 through 144. No protein was detected in homozygous mutant mice by Western blot analysis. (J:58332)