Exon 1被替换了通过同源重组插入的诺姆西林选择座。野生型蛋白在纯合突变胚胎的 Western blot 分析中未检测到。(来源:J:79847)
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Exon 1 was replaced with a neomycin selection cassette inserted by homologous recombination. Normal protein was undetected by Western blot analysis of homozygous mutant embryos. (J:79847)