一段包含137bp的4号外显子、4号内含子以及5号全部外显子的片段被替换为了一个可选择性标记的载体。通过使用氨基酸端和羧基端抗体的 Western blot 分析,对纯合突变小鼠的蛋白质检测结果为阴性。(来源:J:79604)
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A fragment encompassing 137 bp of exon 4, intron 4, and all of exon 5 was replaced with a floxed selection cassette. Protein was undetected in homozygous mutant mice by Western blot analysis using amino- and carboxyl-terminal antibodies. (J:79604)