在C2A域通过同源重组引入了天冬氨酸到天冬酰胺的错义突变(D232N)。对纯合突变动物大脑匀浆的 Western blot 检测确认了蛋白表达的存在。(来源:J:79208)
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An aspartate to asparagine missense mutation (D232N) was introduced into the C2A domain via homologous recombination. Western blot of brain homogenates from homozygous mutant animals verified the presence of protein expression. (J:79208)