Igh^tm3Cgn

The DQ52 element of Igh-D was disrupted by the insertion of a loxP site flanked neomycin resistance gene cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Ightm6Cgn, this allele contains an unmutated Igh-V11 sequence. The Igh intron enhancer (Rr150) was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. (J:76117)