通过同源重组,该基因的整个编码区域被PGK-neo cassette替换。通过E15同源纯合突变胚胎的蛋白质提取物进行 Western blot 分析,证实了基因表达的缺失。(来源:J:70735)
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The entire coding region of the gene was replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression was verified by Western blot analysis of protein extracts from E15 homozygous mutant embryos. (J:70735)