5'启动子和exons 1和2的部分被通过同源重组替换为诺卡因抗性基因座。在纯合突变动物的棒状细胞中,基因表达无法通过 Western blot 分析检测到。(来源:J:43294)
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Part of the 5' promoter and exons 1 and 2 were replaced with a neomycin resistance cassette via homologous recombination. Gene expression was undetectable in rod cells of homozygous mutant animals as shown by Western blot analysis. (J:43294)