Eif2s1^tm1Rjk

A serine to alanine substitution at a phosphorylation site at position 51 (S51A) of exon 2 was introduced, and a floxed neomycin resistance cassette was inserted into the upstream intron for positive selection. The floxed neo cassette was removed by cre-mediated recombination leaving behind a single loxP site and the S51A mutation. Western blot analysis of mouse embryonic fibroblasts derived from homozygous mutant animals confirmed expression of the mutant allele. (J:70004)