IS3区域的一部分序列被替换为包含GFP和neo的载体。通过对野生型小鼠脑组织进行 Western blot 分析,未检测到该蛋白。(来源:J:71480)
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Sequence encoding a portion of the IS3 region was replaced with a cassette containing GFP and neo. Protein was undetected by Western blot analysis of brain tissue obtained from homozygous mutant mice. (J:71480)