在同源重组过程中,1-12号外显子被删除,并被抗氨苄青霉素抗性基因替换。通过胚胎蛋白提取物的 Western blot 分析,证实了纯合突变动物中基因表达的缺失。(来源:J:68368)
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Exon 1-12 were deleted and replaced with a neomycin resistance gene via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of embryonic protein extracts. (J:68368)