在Exon 2,包含起始密码子的位置,被替换了抗氨苄青霉素选择标记。对成年纯合小鼠脑组织的 Western blot 分析显示,这个等位基因没有检测到可识别的蛋白质表达。(来源:J:71828)
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Exon 2, which contains the initiation codon, was replaced with a neomycin selection cassette. Western blot analysis on brain extracts derived from adult homozygous mice confirmed that no detectable protein was made from this allele. (J:71828)