Sod1^tm1Dkd

The gene was disrupted by replacement of exon 5 and flanking intronic sequences with a neomycin resistance cassette via homologous recombination. The mutation deletes C-terminal amino acids 120-154 which constitute the structure and function of the active site channel. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of heart tissue. (J:60470)