Hdac9^tm1Eno

Most of exon 4 and all of exon 5 were replaced by a cassette containing lacZ and neo inserted by homologous recombination. The deleted region encoded amino acids 99 through 176, encompassing the MEF2 binding domain, thus affecting the predominate MEF2-interacting transcriptional repressor (MITR) splice form. RT-PCR and sequence analysis of mutant mice identified aberrant transcript in which the inserted cassette was excised and exon 3 had spliced to exon 6. This alternate splice pattern introduced two stop codons immediately downstream of exon 3, and therefore precluded the translation of normal protein. (J:78734)