内源性位点通过两轮同源重组,将一个含诺卡因选择标记的floxed Neo cassette插入第2 intron,随后是带有floxed puromycin抗性基因及lacZ的3'非翻译区的 cassette。通过Northern和Western印迹分析,确认了这个等位基因产生转录本和蛋白的活性。(来源:J:78711)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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