在第5外显子的第150-155位插入了lacZ-neo cassette,并通过同源重组的方式导入。对纯合突变动物脾组织的 Western blot 检测确认了基因表达的缺失。(来源:J:69806)
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A lacZ-neo cassette was fused in-frame to amino acids 150-155 of exon 5 and introduced via homologous recombination. Western blot analysis of spleen tissue from homozygous mutant animals confirmed the absence of gene expression. (J:69806)