这个基因通过将exon 1替换为一个内切lacZ-neo插件,发生了突变。零等位基因通过北向印迹、RT-PCR和 Western blot在来自纯合突变动物的脑组织样本中的分析得到了确认。(来源:J:69585)
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The gene was disrupted by replacement of exon 1 with an in-frame lacZ-neo cassette. The null allele was confirmed by Northern blot, RT-PCR, and Western blot analysis of brain samples taken from homozygous mutant animals. (J:69585)