使用了cos.6I/LnJ-4的cosmid克隆,它带有鼠源Prnp基因。cosmid Prnp序列来自Inbred品系I/LnJ,该品系携带有Prnpb等位基因(第189位的缬氨酸)。通过转基因动物的RT-PCR分析来验证转基因表达。(来源:J:11316)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count