通过同源重组,将新霉素选择性载体插入到了exon 3的近端。ES细胞的Northern blot分析显示,正常长度的转录本大约以5%的野生型水平存在。后续序列分析确定了新霉素载体和exon 3之间存在一个切割位点,从而允许了少量正常剪接的发生。(来源:J:78546, J:79389)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入
--
1
5
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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