这个基因通过将120bp的exon1开头部分替换为诺卡因抗性基因座,使其失活。对同源突变动物肺、脾和肾的 Western blot 检测确认了这一纯合突变基因型。(来源:J:68125)
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The gene was disrupted by replacing the first 120 bp of exon 1 with a neomycin resistance cassette. Western blot analysis of lung, spleen, and kidney samples from homozygous mutant animals verified the null allele. (J:68125)