Adra1a^tm1Pcs

Exon 1 and 415 bp of intron 1 were replaced with a cassette containing lacZ and neo genes. The deleted region encoded the receptor and transmembrane regions 1 through 5. The absence of transcript was confirmed by both RT-PCR analysis and RNAse protection. Binding assays showed adrenergic receptor binding to be reduced by approximately 50% in brain and kidney tissues and by 70% in heart tissue, all obtained from homozygous mutant mice. Expression of beta-galactosidase was driven by the endogenous promoter. (J:77732)