将31个氨基酸末端激酶域的序列替换成诺卡因选择标记座,对肌肉匀浆进行 Western blot 分析,结果显示纯合突变小鼠中未检测到正常蛋白(来源:J:67575)
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Sequence encoding 31 residues of the amino terminal kinase domain were replaced with a neomycin selection cassette. Western blot analysis of muscle lysates confirmed the absence of normal protein in homozygous mutant mice. (J:67575)