第二外显子编码的第一个假膜蛋白域被替换为一个插入了IRE-Sos序列的LacZ,后面跟着抗氨苄青霉素抗性基因。 Western blot结果显示,纯合突变小鼠睾丸中没有检测到蛋白。(来源:J:72046)
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The second exon coding the first putative transmembrane domain was replaced with an IRES-LacZ sequence followed by the neomycin resistance gene. Western blotting confirmed absence of protein from testis of homozygous mutant mice. (J:72046)