一段人类Il6st的cDNA片段,其中第759位的酪氨酸被替换为苯丙氨酸(Y759F),被用来替换小鼠基因相应区域。下游插入了抗氨苄青霉素抗性基因,用于阳性筛选。通过流式细胞术分析,结果显示突变蛋白在B细胞和T细胞中的表达水平与野生型相当。(来源:J:59922)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
核苷酸替换
--
1
7
21

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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