通过同源重组,将一个PGK-neo cassette插入了内源性基因座的第4外显子。 Western blot结果显示,已确认没有检测到这两种共有的4号外显子形式。(来源:J:71955)
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A PGK-neo cassette was inserted into exon 4 of the endogenous locus by homologous recombination. Western blot analysis confirmed the absence of both known isoforms, to which exon 4 is common. (J:71955)