这个基因通过同源重组的方式,用诺卡因抗性基因 cassette 替换了exon 1,导致其失活。对纯合突变动物脾细胞的 Western blot 检测确认了该基因没有表达(来源:J:60738)。
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The gene was disrupted by replacement of exon 1 with a neomycin resistance cassette via homologous recombination. Western blot analysis of splenocytes from homozygous mutant animals confirmed the absence of gene expression. (J:60738)