体外表达的Cre重组酶导致了包含3、4号外显子全部和5号外显子部分的含floxed序列片段的切除。在杂合突变小鼠的脑组织中,通过 Western blot 分析未检测到蛋白质。(来源:J:71968)
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The in vitro expression of cre recombinase resulted in the excision of a floxed fragment containing all of exons 3 and 4 and a portion of exon 5. Protein was undetected in homozygous mutant mice by Western blot analysis of brain tissue. (J:71968)