通过同源重组,将exons 1和2替换为带有IRES-lacZ-floxed-neo元件。在正确靶向的ES细胞中,通过短暂表达Cre重组酶移除了floxed neo cassette。通过抗POMA抗体检测野生型和纯合突变动物后脑和前脑提取物中的基因表达,未检测到Nova1特异性带,这证实了突变体中基因不表达(来源:J:60771)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
--
1
--
10

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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