Esr2^tm1Mma

The gene was disrupted by insertion of a neomycin resistance cassette into exon 3 encoding the first zinc finger domain via homologous recombination. RT-PCR analysis of ovary mRNA from mutant animals detected 3 faint RNA species all lacking exon 3. Immunohistochemical analysis of ovary sections from homozygous mutant animals did not detect protein product. (J:64426)