Tg(Nes-cre)2Wme

The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included downstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of the loxP-flanked allele occurs, and it may be detected in all adult organs including germ line cells. (J:67926)