一段包含exons 1和2的3kb基因区段通过同源重组被PGK-neo cassette替换。纯合突变体在E12.5日胚胎的 Western blot分析中显示无基因表达(来源:J:28571)。
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A 3 kb region of the gene containing exons 1 and 2 was replaced with a PGK-neo cassette via homologous recombination. Homozygous mutants lack gene expression as demonstrated by Western blot analysis of day E12.5 embryos. (J:28571)