Tg(BLG-Mdm2)1Caf

A full-length Mdm2 coding sequence (including introns 7 and 8) fused to the bovine beta-lactoglobulin (BLG) promoter, active in mammary glands, was used as the transgene. RNase protection assay of total RNA from mid-lactation mammary glands confirmed expression of the transgene intransgenic animals and detected both spliced and unspliced forms. Overexpression of the transgene was estimated to be 10-30 times that of the endogenous gene. (J:72124)