将编码膜段3和4的序列删除,并通过同源重组替换为PGK-neo cassette。通过RT-PCR分析和对新生动物的整个大脑RNA和突触囊泡中的 Western blot,对纯合突变动物的基因表达缺失进行了确认。使用来自突触囊泡的蛋白质进行的结合实验表明,这些纯合突变体没有结合活性。(来源:J:45184)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129X1/SvJ
Targeted
插入,基因内删除
--
1
1
9

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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