编码第1到88个氨基酸的外显子被替换成诺卡因选择标记。通过 Western blot 对大脑、肺和肝脏组织的分析,证实了纯合突变小鼠中无蛋白质表达。(来源:J:65264)
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The exon encoding amino acids 1 through 88 was replaced with a neomycin selection cassette. The absence of protein in homozygous mutant mice was verifed by Western blot analysis of brain, lung, and liver tissue. (J:65264)