在 intron 3 中引入了 loxP 立场,然后通过同源重组将一个含 floxed PGK-neo-tk 嵌入式元件插入到 intron 4。之后,通过转暂时表达 Cre 重组酶,正确靶向的细胞中去除了这个 PGK-neo-tk 嵌合子,留下 loxP 立场环绕在 exon 4 之外。 (来源:J:48085)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
插入
--
1
--
29

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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