整个基因通过同源重组被一个内切lacZ-PGK-neo cassette替换。通过Northern blot对纯合突变动物各组织的基因表达缺失进行了确认。对来自纯合突变体的胚胎成纤维细胞进行 Western blot 检测并未发现蛋白质产物。在突变胚胎中,通过X-gal染色观察到了lacZ的表达。(来源:J:53906)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S2/SvPas
Targeted
插入,基因内删除
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1
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22

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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