Tg(Bglap-ESR1-delta3)FVld

The human ESR1 gene carrying a natural deletion of exon 3 due to alternative splicing was used as the transgene. 200 kb of truncated rat Bglap promoter sequences was used to drive expression of the transgene. The promoter truncation results in loss of tissue-specific expression, therefore expression of the transgene is found in most tissues. The exon 3 deletion results in a dominant negative form of the protein lacking the second zinc finger of the DNA-binding domain, and acts as a repressor of ESR1. (J:77730)