Tg(GAS)1Tcw

The human gastrin gene engineered with a stop codon after glycine 72 and under the control of the mouse metallothionein promoter was used as the transgene. The transgene was verified by Southern blot analysis using a human gastrin exon 2 probe. Northern blot analysis detected transgene expression in kidney, liver, colon, stomach, and pancreas of transgenic animals using the exon 2 probe. RT-PCR analysis confirmed the expression of transcripts truncated after codon 72 in the colon of transgenic animals. (J:54869)