Cxcl13^tm1Cys

Base pairs 18 through 116 (exon 2) were replaced by a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice. (J:78282)